Recombinant DNA Technology/Genetic Engineering (10 days)
- Restriction digestion of DNA and Plasmids & gel electrophoresis
- Ligation and gel electrophoresis
- Conjugation
- Gene Cloning
- Preparation of Competent cells by Calcium chloride method
- Transformation of Recombinant DNA into appropriate host cells
- Screening for Recombinants
- RAPD Analysis
- DNA Gel elution techniques
- Amplification of Genomic DNA by Random primers using PCR
- Agarose gel electrophoresis of RAPD samples
- Southern Blotting
- RFLP Analysis